Inoculation - Medicine

What is Inoculation?

Inoculation Definition - Inoculation is the set of different methods of artificially inducing immunity against some infectious diseases. This practice was imported from the western world to the eastern world, its origin is unknown in different parts of Africa and it will vary potentially. The terms vaccination, immunization and inoculation meaning are the same, often used synonymously, but there is some important difference among them. 

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Inoculation meaning in microbiology is that the introduction of micro-organism into the environment where they can grow and reproduce, it is the direct transfer from the culture of microorganisms to the inoculation needle (from one substance to another substance).

Types of Inoculation Media 

Different types of inoculation media are agar plates, broth culture, slant culture, plate culture, stab culture, etc., which has been discussed in detail below. 

Agar Plates

It is one of the most common media used for growing bacteria and some of the other microorganisms, the mixture of agar and nutrients are required for bacterial growth. This mixture is poured into the circular Petri dishes, where the solidification of the agar solution takes place, after that, inoculation of a solution containing microorganisms on these plates with the help of streaking.   

A small streaking loop is a dip into a solution, which contains bacterial cells that are used to streak onto the plates with the bacteria and these plates are stored at the proper temperature. liquid media can be inoculated in the suspensions of bacteria to grow and reproduce.

Broth Culture

Inoculation injection used to inject a culture of sterile broth, burning the open end of the broth will keep the need for ingestion sterile. The needle broth is removed so that the needle tip is immersed while maintaining the original position of the needle.

Careful rotation of the needle can aid in the insertion of a microorganism from the needle into a sterile broth. The custom of the vaccinated broth was then removed from the needle. With the help of the Aseptic method used in the open end of the broth culture to prevent contamination, and the needle burns sterilization.

Slant Culture

Inject the slant culture in the form of fishtail inoculation using an inoculation injection needle. After transferring microorganisms from the original microbial culture to the inoculation needle the sterile culture is not included. By the end of the opening of the unsealed slant culture the flames are already burning.

The position of the slide is that it moves the needle until the tip of the touch needle meets the top of the sterile media base. A zigzag pattern is formed on the surface of the agar when the injection needle injects the agar by deceiving the media. To remove the inoculation injection needle, we use an aseptic procedure.

Plate Culture

The custom installation of the plate uses the streaming process to create a plate of the streak. The needle inserted across the plate in a controlled manner, after lifting the lid to move it over the barren agar plate. Microbial aerosols are created from the tipping of the inoculation tip on the sides of the agar plate. The injection needle is removed from the centre of the agar plate that is concentrated and ignited.

Stab Culture

In establishing a culture of piercing, an injection needle is an important tool. The sterilization of sterile sterilization is complete and the end of the needle is opened. The tip and needle of the needle are pushed into the stabbing sources until the needle reaches 0.5 inches from the bottom of the stabbing machine. The inoculation injection is removed from the media in the same direction as the insertion device into the stabilizer sources to prevent a loose impact that could disrupt the culture. We can kill the needle with the help of flames.

What is Inoculation in Microbiology? 

In microbiology, the meaning of inoculation is somewhat different from the common term such as health, vaccines, and immunology. Inoculation in microbiology is the study of the introduction of microorganisms into environments where they can grow and reproduce.

For example, inoculation is the method of adding certain types of nutrients or chemicals into the suspension of bacteria, we can also say that inoculating a suspension with a particular nutrient or chemical. 

Difference Between Inoculation and Vaccination 

Mostly, there’s no difference between inoculation vs vaccination, one of them ultimately is an act and the other is the process that spurs immunity. By going through the definition, you can understand it in a better way. 

Inoculation is the process of introducing a vaccine into the person’s body, where it can be used in another context. Such as, when the culture is inoculated in the body fluids of humans (like nasal swab) to test the presence of bacterium or virus.

Vaccination is the act of injecting a vaccine into the human body to produce immunity in one of the diseases. Vaccines are usually made with injections but can also be administered orally through the nose for protection.

Did You Know? 

Where was inoculation originated? Inoculation was introduced as a method for the prevention of disease like smallpox by the deliberate transfer of material from smallpox postulate into the skin. The result of it produced less severe infection than the naturally acquired smallpox, but it would induce immunity to it. Smallpox inoculation was the first method for smallpox prevention, it is also known as variolation. Some of the ancient origins of the inoculation technique are known in India and China. 

FAQs (Frequently Asked Questions)

Q1. What do You Mean by Inoculation Needle?

Ans: The vaccine needle is a laboratory device useful in the field of microbiology to transmit and inject living germs. It is one of the most common tools in the natural laboratory. It has two disposable or disposable types. A reusable inoculation needle made of nichrome or platinum wire attached to a metal handle and a disposable inoculation injection is made of plastic resin.

Q2. When was the Inoculation Process Banned?

Ans. The government banned the process of inoculation in 1840 because it prevented the previously strong opposition to it from the use of vaccination. 

Q3. Who Came up With the Inoculation?

Ans. Inoculation was introduced by Edward Jenner in the west in 1796 when he inoculated a 13-year-old boy with the vaccinia virus (cowpox). He demonstrated immunity to smallpox, the first smallpox vaccine was developed in 1978.