Question

Proteins do not respond to:
(A) Biuret test
(B) Heller’s ring test
(C) Ninhydrin test
(D) Lucas test

Answer 1

Hint: Proteins have a high concentration of long chain polymer cells made up of $\alpha - $amino acids. Proteins are part of the cells and are therefore present in living bodies. Proteins contain carbon, hydrogen, nitrogen, oxygen and sometimes phosphorus and sulphur.

Complete Step by Step Solution:
Biuret Tests: Compounds with peptide interactions perform this experiment. Protein polypeptides and amino acids are bound together by peptide bonds. The alkaline protein solution is treated with a drop of aqueous copper sulphate where a violet bluish colour is obtained.

Heller's ring tests: Heller's tests usually use concentrated nitric acid to convert proteins. Tests are performed for clinical purposes to detect abnormal proteins in biological fluids, including urine. Heller testing is a type of rain test where rain is brought in by denaturation fluctuations.

Ninhydrin Testing: Proteins mixed with the pyridine solution of ninhydrin provide a colour solution ranging from deep blue to deep pink and sometimes even red in some cases. Ninhydrin solution is prepared by dissolving ninhydrin in distilled water. But this ninhydrin solution is unstable and can be stored for two days.

Lucas test: Lucas test is used to separate and separate primary, secondary and third alcoholic beverages using anhydrous zinc chloride solution in concentrated hydrochloric acid.

Note: Some protein tests are: Xanthoproteic test, Millions test. Xanthoproteic Tests Proteins in nitric acid treatment give a yellow or orange colour. Concentrated nitric acid is used for nitrate. In nitric acid treatment, the protein gives off a yellow liquid that turns orange in the treatment of alkali. And millions of tests are a phenolic group of tyrosine proteins that react with mercuric sulphate in the presence of sodium nitrite and sulfuric acid to give it a reddish colour. The Milon test is based on proteins containing phenolic amino acids.