Question

A form of partition, adsorption, ion exchange or affinity chromatography in which one phase is liquid (aqueous) flowing down a column packed with the second phase, a solid is called _______.
A. Partition Chromatography
B. Column Chromatography
C. Thin layer Chromatography
D. Adsorption Chromatography

Answer 1

Hint: Chromatography is a method used to separate mixtures into its components. It is also used to test the purity of the compounds. It was described by Tswett in and was first used for separation of coloured substances found in plants.

Step-by-Step Solution-
Partition chromatography- is based on the differences in tendencies of substances to distribute or partition between two phases.
In this process, the components undergo continuous differential partition between the moving and stationary phase.
 The best example of partition chromatography is paper chromatography.
Column chromatography- is used to separate components of a mixture using a column of suitable adsorbent( which is a solid) in a burette or glass tube.
The liquid mixture is placed at the top of the column and it flows down the column slowly.
The component with high adsorption gets adsorbed at the top while the others are adsorbed at different heights lower than the highest one.
Thin layer chromatography- involves separation of substances of a mixture over a thin layer of an adsorbent coated over a plate called chroma plate which is then placed in a container containing solvent. This solvent moves up the plate and the components of the mixture move up along the plate to different distances.
Adsorption chromatography- is based on the fact that different compounds are adsorbed on an adsorbent to different degrees. A mobile phase is allowed to move over a stationary one and the components with different adsorption tendencies

Answer-Hence the correct answer is B.

Note: The uses of chromatography are-
- It is used in bio analytical chemistry to separate, isolate and purify proteins.
- To isolate chiral compounds
- To test trace amounts of contaminants in a product.