
Cell organelles can be separated by method of
A) Autoradiography
B) Microtomy
C) Differential centrifugation
D) X-ray diffraction
Answer
411.6k+ views
Hint: Cell organelles are the cellular elements. These cell organelles include membrane-bound and non-membrane-bound organelles that are found inside the cells and have different structures and functions. For the cell's normal functioning, they organise and act effectively.
Complete answer:
The basic principle involved in sceptrin of orangelo is sedimentation of particles in a suspension by centrifugal force. Centrifugation is a common process in microbiology and cytology used to separate many organelles from whole cells to study about its characteristics and features.
Process of centrifugation involves:
1. Sample cell is first homogenized so that it can break the cell membranes and cell contents are mixed up.
2. The homogenate is processed to repeated centrifugations, each time removing the pellet and increasing the centrifugal force.
3. Finally, purification may be done through equilibrium sedimentation, and the desired layer is extracted for analysis of specific parts of cells.
In a centrifugation, the particles sediment at different rates when an accelerating force is subjected. Organelles can be separated from cell homogenate through differential centrifugation.
So, option C is the correct answer.
Note:
The rate of sedimentation depends upon the size of the particles, its shape and density. So different particles or organelle can be separated easily on the basis of size, weight.
Complete answer:
The basic principle involved in sceptrin of orangelo is sedimentation of particles in a suspension by centrifugal force. Centrifugation is a common process in microbiology and cytology used to separate many organelles from whole cells to study about its characteristics and features.
Process of centrifugation involves:
1. Sample cell is first homogenized so that it can break the cell membranes and cell contents are mixed up.
2. The homogenate is processed to repeated centrifugations, each time removing the pellet and increasing the centrifugal force.
3. Finally, purification may be done through equilibrium sedimentation, and the desired layer is extracted for analysis of specific parts of cells.
In a centrifugation, the particles sediment at different rates when an accelerating force is subjected. Organelles can be separated from cell homogenate through differential centrifugation.
So, option C is the correct answer.
Note:
The rate of sedimentation depends upon the size of the particles, its shape and density. So different particles or organelle can be separated easily on the basis of size, weight.
Recently Updated Pages
Master Class 12 Economics: Engaging Questions & Answers for Success

Master Class 12 Maths: Engaging Questions & Answers for Success

Master Class 12 Biology: Engaging Questions & Answers for Success

Master Class 12 Physics: Engaging Questions & Answers for Success

Master Class 12 Business Studies: Engaging Questions & Answers for Success

Master Class 12 English: Engaging Questions & Answers for Success

Trending doubts
Who is Mukesh What is his dream Why does it look like class 12 english CBSE

Who was RajKumar Shukla Why did he come to Lucknow class 12 english CBSE

The word Maasai is derived from the word Maa Maasai class 12 social science CBSE

What is the Full Form of PVC, PET, HDPE, LDPE, PP and PS ?

Why is the cell called the structural and functional class 12 biology CBSE

Which country did Danny Casey play for class 12 english CBSE
