Answer
425.1k+ views
Hint: The sticky or blunt end of a fragmented DNA molecule depends on the enzyme which is used to cut the DNA
Complete answer:When both strands of DNA terminate in a base pair it is called blunt end whereas when these ends are created by various overhangs are called non blunt ends.
1. Non blunt ends are also called Sticky ends or cohesive ends when these overhangs are longer overhangs.
2. The stretch of unpaired nucleotides in the end of DNA is termed as overhang. These unpaired nucleotides are in either strands which create 3’ or 5’ overhangs.
3. There are two types of restriction enzymes viz, restriction endonucleases or exonucleases.
4. Restriction enzymes leave single stranded portions at the ends because it cuts the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
5. When the DNA is cut with a sticky end, it is most probably due to restriction of endonuclease enzymes.
6. When this enzyme cuts the pair of DNA strands it forms four base pairs, which create a four-base 5' overhang in one molecule and a complementary 5' overhang in the other.
7. These ends can be easily joined back together by a ligase.
8. Sticky ends form hydrogen bonds with their complementary cut counterparts hence the name, sticky ends.
Therefore, the correct answer is option C) Unpaired bases.
Note: When one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.
Complete answer:When both strands of DNA terminate in a base pair it is called blunt end whereas when these ends are created by various overhangs are called non blunt ends.
1. Non blunt ends are also called Sticky ends or cohesive ends when these overhangs are longer overhangs.
2. The stretch of unpaired nucleotides in the end of DNA is termed as overhang. These unpaired nucleotides are in either strands which create 3’ or 5’ overhangs.
3. There are two types of restriction enzymes viz, restriction endonucleases or exonucleases.
4. Restriction enzymes leave single stranded portions at the ends because it cuts the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
5. When the DNA is cut with a sticky end, it is most probably due to restriction of endonuclease enzymes.
6. When this enzyme cuts the pair of DNA strands it forms four base pairs, which create a four-base 5' overhang in one molecule and a complementary 5' overhang in the other.
7. These ends can be easily joined back together by a ligase.
8. Sticky ends form hydrogen bonds with their complementary cut counterparts hence the name, sticky ends.
Therefore, the correct answer is option C) Unpaired bases.
Note: When one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.
Recently Updated Pages
Integrate the following function sqrtsin 2xcos 2x-class-11-maths-ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
If X is a Poisson variate such that Pleft X2 right9pleft class 11 maths ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
If the value of y log left tan x right findfracdyd-class-11-maths-ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
If a and b are unit vectors and left a+b right1the-class-11-maths-ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Find the value of x + y+ z if it is given that tan-class-11-maths-ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Find the value of integral int0pi 2dfracsin 2x1+2c-class-11-maths-ICSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Trending doubts
Write a letter to the principal requesting him to grant class 10 english CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Difference between Prokaryotic cell and Eukaryotic class 11 biology CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Give 10 examples for herbs , shrubs , climbers , creepers
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Change the following sentences into negative and interrogative class 10 english CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Difference Between Plant Cell and Animal Cell
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Fill the blanks with proper collective nouns 1 A of class 10 english CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
The Equation xxx + 2 is Satisfied when x is Equal to Class 10 Maths
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
Who is the executive head of the government APresident class 10 social science CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)
What organs are located on the left side of your body class 11 biology CBSE
![arrow-right](/cdn/images/seo-templates/arrow-right.png)