Question

Name the bacterium that yields a thermostable DNA polymerase.

Answer 1

Hint: DNA polymerase isolated from a type of bacteria that can withstand high temperatures, one of the few thermophilic bacteria belonging to the Deinococcus-Thermus group. Because it is used in DNA polymerase chain reaction (PCR) amplification technology, it is the source of the thermally stable enzyme Taq DNA polymerase, one of the most important enzymes in molecular biology.

Complete answer:
Enzymes isolated from Thermus aquaticus
> Aldolase
> RNA polymerase
> Taq 1 restriction enzyme
> DNA polymerase
Taq polymerase is an enzyme found in the bacteria Thermus aquaticus. It is a class 4 enzyme, a class of lyase enzymes. Lyase is an enzyme that catalyzes the dissolution of a substrate and creates double bonds.
Taq polymerase is a DNA polymerase that allows bacteria to proliferate at high ambient temperatures due to its thermal stability. These properties make Taq polymerase an important commodity.
Each organism contains the enzyme DNA polymerase, which is necessary for replication, but due to its protein composition, it denatures most at high temperatures. The DNA polymerase obtained from Thermus aquaticus remains stable even at very high temperatures. Because of this stability, it can be used for a process known as polymerase chain reaction (PCR).
This is why Taq polymerase is so important. It can be used many times in this chain reaction.

Note:
The PCR technology was developed by Carey Mullis in 1983. Ten years later, he was awarded the Nobel Prize for this achievement.
Chien et al first isolated Taq polymerase. It is successfully replicated in one E. coli strain and expressed in another organism. PCR is now widely used for DNA replication and manipulation, analysis of genetic functions and mutations, diagnosis of genetic and infectious diseases, and identification of genetic diseases.