Question

In Tobacco callus, which one shall induce shoot differentiation in combination of auxin and cytokinin
a. Higher concentration of cytokinin and lower concentration of auxin
b. Lower concentration of cytokinin and higher concentration of auxin
c. Only cytokinin and no auxin
d. Only auxin and no cytokinin

Answer 1

Hint: The plant hormone that helps in the promotion of the cell elongation and produced in the stem tip and roots. The movement of the auxin is through the darker side of the plant. It promotes cell division and causes plant cell elongation. The plant will grow towards the light in the presence of auxin.

Complete answer:
The root and shoot that is initiated by the culture on media and ratio of auxin and cytokinin present in tobacco callus. The transfer of callus to the media takes place from a high concentration of cytokinin and low concentration of auxin for shoot development. Once the shoot development is completed then it is transferred to a media of roots which contains high auxin concentration and low cytokinin concentration for rooting.

The hardening treatment starts after root and shoots growth in which the plant will grow under available temperature in the fields and prepare them for growth. From the plant media, the plantlets are removed and put it in the soil. For example – traits propagation that is economically important and transgenes introduction.

The plants that grow should contain cytokinin and auxin both of which promote growth and maintain the concentration of themselves properly. So option (C) and (D) are incorrect.

Hence, the correct answer is option (A).

Note: The generation of callus is mainly done by auxin and cytokinin which helps in the callus induction at the molecular level. The callus culture is used for secondary metabolites production, therapeutic antibodies production, etc. The callus tissue of tobacco was measured without the organogenesis process and with the production of growth and nicotine.