Question

Chargaff’s rule is applicable to:
A. single stranded RNA
B. single stranded DNA and RNA
C. single stranded DNA
D. double stranded DNA

Answer 1

Hint: Adenine, thymine, guanine, and cytosine are not present in equal amounts and differ between species. They can be utilized to distinguish between different species since they are constant among members of the same species.

Complete step by step solution:
A principle that states that the numbers of bases A and T and G and C are always equal in DNA. (Adenine, Thymine, Guanine, and Cytosine make up the letter sequence.) named in honor of the outstanding Austrian-American biochemist Erwin Chargaff (1905–2002), who formulated this rule.
According to the Chargaff's rule, DNA contains a 1:1 ratio of purines to pyrimidines. More specifically, the amounts of guanine and adenine should be equal to cytosine and thymine, respectively. It offers the framework for base pairing. This rule can be used to both determine the DNA strand length and if a base is present in the molecule. The reason Chargaff's principles are significant is that they hint at a sort of "grammar of biology," or a set of unspoken laws that control DNA shape. This grammar should manifest itself as universal DNA patterns that apply to all animals.
The results of Chargaff's early work with paper chromatography and UV spectrophotometry directly influenced later understandings of the DNA molecule's structure, as well as how DNA replicates and encodes genetic information.
Both of the DNA's strands display this pattern. Because DNA and single-stranded RNA lack complementary bases, Chargaff's rule does not apply to them.
So, option (D) is correct.

Note:
In his lifetime, Erwin Chargaff put forth two fundamental guidelines that were known as Chargaff's rules. The earliest and most well-known accomplishment was demonstrating that the ratio of guanine to cytosine and the ratio of adenine to thymine in natural DNA is equal.