Question

Application of adsorption chromatography is/are:
A. separation of relatively non-polar, water-insoluble compounds.
B. separate of isomers
C. purification of antibody fragments
D. all of the above

Answer 1

Hint: Adsorption is the phenomenon in which the substance is adsorbed only on the surface of the adsorbent and not inside the surface. It has many applications such as gas masks, control of humidity, chromatography, etc.

Complete answer:
- In the given question we have to tell whether the given application of the adsorption chromatography is correct or not.
- As we know that the absorption is the accumulation of the adsorbent on the surface rather than the bulk is known as adsorption.
- Whereas absorption is the phenomenon in which the substance is absorbed in the bulk inside the absorbent.
- So, adsorption has much application due to its ability to the substance on its surface such as
- We can separate the molecules which are nonpolar because as we know that water is a polar liquid so it can dissolve only polar molecules.
- So, the non-polar molecules will be insoluble in the water and they can be absorbed by the adsorbent i.e. by paper chromatography on its surface.
- So, option A is the correct statement.
- Similarly, we can separate different isomers based on their solubility in the solvent and can be adsorbed by the adsorbent which has more interaction with the substance.
- Adsorption chromatography is the method in which the mobile substance either gas or liquid is adsorbed on the stationary adsorbent.
- So, option B is also the correct answer.
- Now, the purification antibody fragment can also be done by using the process of adsorption chromatography.
- For example, the separation of the antibodies from the serum is done based on the charge present on them.
- So, option C is also the correct statement.

Therefore, Option D is the correct answer because all the statements are correct.

Note:
The substance on which the substance is adsorbed is known as adsorbent whereas the substance which is adsorbed is known as adsorbent. There are two types of adsorption i.e. physical and chemical adsorption.