VerifiedHint: Consider the purpose of PCR and its limitations.
Complete step-by-step Answer: PCR stands for polymerase chain reaction. It is a technique used to amplify DNA, which means making many copies of a specific DNA sequence. PCR is a very powerful tool that can be used for a variety of purposes, including:
Analyzing DNA from ancient remains
Analyzing the genome of a virus
Determining the DNA of a hair sample found at a crime scene
However, PCR cannot be used to transfer DNA from a plasmid containing GFP into E. coli. This is because PCR is used to amplify DNA, not to insert DNA into cells.
To insert DNA into cells, a technique called transformation is used. Transformation is a process in which bacteria take up DNA from the surrounding environment.
To transform E. coli with a plasmid containing GFP, the plasmid DNA is first mixed with the E. coli cells. The E. coli cells are then treated with heat shock, which makes their cell membranes more permeable. This allows the plasmid DNA to enter the E. coli cells.
Once the plasmid DNA is inside the E. coli cells, it can replicate and be expressed. This means that the E. coli cells will produce the GFP protein, which will make them glow green
Hence the correct answer is Option D
Note: PCR is a very versatile technique that can be used for a variety of purposes, but it is important to understand its limitations. PCR cannot be used to insert DNA into cells, so it cannot be used to transform E. coli with a plasmid containing GFP.
