Question

Which is not a hydrolase?
A) Protease
B) Dehydrogenase
C) Sucrase
D) Amylase
E) Esterase

Answer 1

Hint:Hydrolases are the class of enzymes that by reaction with water catalyze the cleavage of a covalent bond, which usually results in the division of a larger molecule into smaller molecules.

Complete answer
To answer this question, we have to know about dehydrogenase. A dehydrogenase is an enzyme that oxidizes a substrate by discarding, in its oxido-reduction reactions, hydrogen atoms [H] instead of oxygen [O], typically a flavin coenzyme recognized as FMN. All the others, though, are hydrolases, since they are involved in hydrolysis.

As they break down large molecules into fragments for synthesis, metabolize waste materials, and provide carbon sources for energy production after which many polymers are transformed to monomers, hydrolases are crucial for the body. In the liver and kidneys, this enzyme is abundant, and small quantities are discovered in many tissues in the body.

In biochemistry, a hydrolase catalyzes the hydrolysis of a chemical bond like the following reaction:
$A-B+H_2O+\rightleftharpoons A-H+B-OH$

Esterases, including such phosphatases, that act on ester bonds, and proteases or peptidases that act on amide bonds in peptides are types of hydrolase. When they take place, some hydrolases might emit photons. As peripheral membrane proteins, a number of hydrolases, especially proteases, are linked to biological membranes or affixed via a single transmembrane helix. Amylase and sucrase act on carbohydrates; amylase catalyzes starch hydrolysis into sugars, while sucrose catalyzes sucrose hydrolysis (fructose and glucose) into its subunits.

Hence, the correct answer is option (D).

Note: In the respiratory chain pathway or the electron transfer chain, dehydrogenase is a flexible enzyme. This enzyme is mainly found to transform ethanol into acetaldehyde in the liver and stomach, a poison that is then subsequently broken down into acetic acid by acetaldehyde dehydrogenase, which can be transformed to carbon dioxide and water.