Question

The process through which the amount of DNA, RNA, and protein can be known at a time is called
A. Autoradiography
B. Tissue culture
C. Cellular fractionation
D. Phase contrast microscopy

Answer 1

Hint: The process of identification of DNA, RNA, and protein at a time use the radiolabeled molecules for tagging. These tags then studied to identify the mentioned molecules.

Complete answer:
1. Autoradiography is a process that includes the use of radiolabeled molecules to identify the biomolecules. For example, radioactive thymine is used for the detection of DNA, radioactive uracil is used to identify the RNA and radioactive sulphur is used for the detection of proteins. First radiolabelling of the biomolecules is done and then these are placed on a surface of the slide and photosensitive emulsions are applied to study the biomolecules.
2. Tissue culture is a method in which culturing of cells, tissues, and organs are done. This is an in vitro technique.
3. Cellular fractioning is a biotechnological method that can be defined as the separation of cellular organelles through sequential homogenization, filtration, and purification method.
4. Phase-contrast microscopy is a modern type of microscopic technique that has the ability to pass light through the specimen according to the density of the cellular compound. It gives a light colour of specimen against a dark background and a dark image of specimen against a light background.

Thus, the correct answer is option A. Autoradiography.

Note: DNA, RNA and proteins can be identified separately by different methods. For example, DNA fingerprinting helps to identify the characteristics of the DNA. Proteins can be separated and identified by the chromatography techniques.