Question

The name of the experiment performed by Lederberg is
A. Replica plating experiment
B. Tissue culture
C. Protoplast fusion
D. Hybridization

Answer 1

Hint: For Mem RS was also known to be Joshua Lederberg. He was a famous American molecular biologist, who was well known for his contributions in microbial genetics, artificial intelligence, and the United States space program. It is a fact that he was only 33 years old when he won the 1958 Nobel Prize in Physiology or Medicine. He was honored by presenting the Nobel Prize for discovering that bacteria can mate and exchange genes.

Complete answer:
-The replica plating experiment is the name given to the Lederberg experiment. They spread the diluted cultures of bacteria on nutrient intermediate plates and gestated till visible bacterial colonies are observed.
-The next step typically involves the process of inversion and pressing of plates over velvet cloth, then the similar material of velvet was moderately pushed over the medium of nutrient plates which contain antibiotics like ampicillin and incubated.
-The next step After the process of incubation is rare ampicillin unaffected gatherings that have formed that can effortlessly thrive in-plane nutrient standard. It gave rise to ampicillin resilient colonies. This procedure is recognized as the process of the replica plating technique.
-The process of the tissue culture includes the development of the particular tissue or cell in an artificial medium, generally separate from the parent organism.
-The process of hybridization includes a combination of two or more varieties to form a hybrid crop.
-The process in which discrete species of plants are made fuse together to form a new hybrid plant that consists of characters of both the plants is called protoplast fusion.
Thus, the correct option is (A) ‘replica plating experiment.’

Note:
The resistance of the colonies in a population of bacteria towards the antibiotics can be shown by growing the resistant colonies on a plane nutrient medium. Thus, we can say that the resistance of the settlements is before their exposure to the antibiotic. However, developing more numbers of plants in less duration and less cost is called tissue culture.