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The main difference between the gram-positive and gram-negative bacteria lies in the composition of
A. Cilia
B. Cell wall
C. Nucleolus
D. Cytoplasm

Last updated date: 13th Jun 2024
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Hint: The primitives of our Earth have a very simple organization. They can carry out the processes with one cell. Bacteria thus are very simple and prokaryotes. All the bacterias have almost the same features except for one or two. The staining process is done to differentiate them.

Complete answer:
Bacterias are known to be the most primitive and simplest creatures. They are prokaryotic means having simple metabolism. They do not have membranes around their cell organelles and the nucleus. They are usually unicellular, that is the single-cell body carries out all the functions including reproduction, circulation, excretion etc.
The bacterial cell wall is composed of peptidoglycan (murein) made of polysaccharide chains. The thickness of this layer differentiated the bacterias into two labels-gram positive and gram-negative.
Gram-positive bacteria have a thick murein layer with no outer membrane, due to this they retain the crystal violet colour in them.
Gram-negatives have the thin murein layers which make them lose the colour on decolourization. Thay have an outer membrane.
Let us see all the options.
Option A: Cilia: These are the protuberances in the body of the bacterias. The motile cilia will help in the movement while the non-motile cilia will serve as a sensory organ. It can be present in both positive and negative bacteria.
Option B: Cell wall: Their cell wall is made of murein but in the gram-positive it is thick and in the gram-negative, it is thinner. Due to this property, the staining is done to differentiate between them.
Option C: Nucleolus: It has the function of rRNA generation and ribosome synthesis in the eukaryotes. The fact being here that there is no nucleolus present in the bacteria as they are prokaryotes.
Option D: Cytoplasm: The matrix of the cell is the cytoplasm in which the organelles in the prokaryotes lie freely. They are not membrane so they roam freely both in the gram-positive and negative bacteria.

So the answer is option B: Cell wall.

Note: It was given by Hans Christian Gram. The experiment was done when he found that the lung of a pneumonia person had taken some different stain. The gram-positive bacterias will show the purple colour while the negative bacteria will show pink colour under the microscope.