Question

Polyacrylamide gel is prepared by mixing acrylamide and ______________ at fixed proportions.
A.Glycol
B.Bisacrylamide
C.Hydrazine
D.None of the above

Answer 1

Hint: Polyacrylamide gel is made by cross-linking of polymers by the process of polymerization. Both the monomers contain the same functional group.

Complete step by step solution:
 Polyacrylamide gel used in electrophoresis are three-dimensional networks, made by the polymerization of acrylamide with the bi-functional reagent N,N’-methylene-bis-acrylamide, which is called as Bisacrylamide at room temperature.
Samples can be anything ranging from proteins or nucleic acids. These may be biologically derived such as prokaryotic cells, eukaryotic cells, viruses, or purified proteins. In case of solid tissues or cells, they are first broken down using a blender using a homogenizer, by sonication using cycling of high pressure and a combination of biological and mechanical techniques.
Polyacrylamide gel electrophoresis is a commonly used technique in microbiology, molecular biology, forensic chemistry, genetics, and biochemistry to separate biological macromolecules, usually, protein and nucleic acids according to their electrophoretic mobility. Electrophoretic mobility is a function of the conformation, charge, and length of the molecule.
Polyacrylamide gel electrophoresis is a commonly used tool used to analyse RNA samples. When polyacrylamide gels are denatured after electrophoresis, it provides information on the composition of the RNA species.

Hence, the correct answer is option B.

Note:
Acrylamide polymer is in a powder state before the addition of water and it is toxic to the human nervous system. Acrylamide is soluble in water and it polymerizes due to the addition of water and forms polyacrylamide.