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Mendel’s principle of segregation is based on separation of alleles during
A. Gamete formation
B. Seed formation
C. Pollination
D. Embryonic development

Answer
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Hint: Mendel’s principle of segregation demonstrates that the two alleles for each trait segregate or separates during the formation of gametes during the formation of gametes during the formation of new zygotes. In this principle it is stated that the alleles will combine at random with other alleles.

Complete answer:
According to Mendel’s law of segregation, a monohybrid cross which occurs during gamete formation, the alleles for each gene segregate from each other which carry only one allele for each gene. The law of segregation states the above demonstrated statement.
Seed formation, pollination and embryonic development is not associated with the Mendel’s law of segregation. Gamete formation is linked to Mendel’s law of segregation as alleles separate from each other and form new zygotes in a plant which helps in formation of new traits due to combination of random alleles. Segregation of genes occurs during the meiosis division in eukaryotic organisms which is a process that produces reproductive cells called gametes. Mendel crossed two heterozygous pea plants which contain two different alleles which encoded the traits in each parental plant which have separated from each other during formation of reproductive cells.

Hence, the correct option is A. Gametes formation.

Note: Mendel gave three major principles of genetics which were the law of dominance, the law of segregation and the law of independent assortment. These all laws of Mendel help us to study evolution and genetics of an organism.