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Uses of Autoclave

Last updated date: 04th Mar 2024
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What is an Autoclave?

Autoclave was invented by Charles Chamber-land in 1879.Autoclave is a machine used in industrial processes and laboratories to perform scientific facts.  Autoclave refers to the use of any equipment or chemical by setting high temperatures and high pressures for a specified period of time. In other words, to perform processes that require the  proper operation.  For this we use autoclave.

Autoclave Parts and Functions: 

Autoclave is also called steam dehydration.  Autoclave is basically a machine that creates a high temperature high pressure environment for a fixed time interval.

  • The most important component in this is the pressure chamber autoclave, which consists of an inner chamber as well as an outer jacket, with a capacity of 100 litres to 3000 litres.

  • Then the main component is the lid of the autoclave which helps to create sterilisation conditions inside the autoclave different from the outside environment.  

  • Evidence It gives information about the temperature specified in the economic zone during sterilisation or serves as a measure of price.  

  • The lid of the autoclave has a whistle similar to that of a pressure cooker, which controls the stains inside.  

  • There are safety valves inside the autoclave which ensures that if the autoclave does not function properly, it will automatically explode to avoid danger.  

  • The electric generator is located under the chamber, which heats the water and generates the part in the inner and outer chamber, as a result of which an electric heating system can be used.  

  • The autoclave has a waste water cooler to remove the water, which helps in cooling the water out.

Working of Autoclave

Working of Autoclave

Autoclave Diagram: 

The simplest form of autoclave is the pressure cooker type or laboratory bench autoclave.  Which consists of the following parts:

  • Pressure Regulating Device

  • Pressure Gauge

  • Safety Valve

  • Autoclave Lid

  • Handles

  • Autoclave Body

  • Steam Release Valve

  • Vacuum Release Valve 

  • Outer stand

Diagram of Autoclave

Diagram of Autoclave

Uses of Autoclave: 

As a result of using autoclave, we can use high temperature and high pressure for proper process, this very useful equipment. Uses of autoclave are as follows

  • Autoclave is done to sterilise bacteria from equipment etc.



  • It can determine properties from all types of solids or liquids.  

  • All the containers used in the laboratory like burettes, pipettes, test tubes, etc., are sterilised using autoclaves.  

  • Other bacterial viruses can also be destroyed from organic waste with the help of autoclave.  

  • Autoclave is used to sterilise all medical instruments, surgical instruments, medical waste, hand gloves, etc., with the help of autoclaves.

Autoclaves are mainly used in microbiology, medical field, veterinary science, artificial manufacturing, dentistry, etc. 

Uses of  Autoclave in Microbiology: 

Cleanliness and determination are important aspects in scientific experiments.  To make all this equipment again clean and germ-free, equipment like autoclave is usually used.

To study microbiology, collect information about all the little creatures in the world.  These creatures are so small that we are not able to see with our own eyes.

Autoclave Uses in Laboratory: 

The equipment used in the laboratory also needs to be purified.  After performing various chemical experiments and before carrying out experiments in the laboratory, it is necessary to ensure that no bacteria, viruses or pesticides of any kind are present in any equipment, for this reason autoclave is necessary in the laboratory.  All the equipment is sterilised with the help of autoclave, which does not result in any other side effects of any kind of process.


Autoclaves are often used to destroy bacterial viruses from gloves, medical equipment, etc.  It works on the principle of heat sterilisation, where as a result of pressure and temperature all the contents present inside can be fixed.  Autoclave is easy to use, but precautions must be observed.  The autoclave should never be overcrowded with too much equipment. 

Solved Questions:

1. What is Autoclave? 

Autoclave is a heated container used for chemical processes and other reactions using high pressure and high temperature which is very strong.

2. On what principle does autoclave work? 

The autoclave works on the principle of moist hot sterilisation, where the part is used to sterilise all the waste material present in the chamber using pressure.

3. What are the types of autoclave? 

Types of autoclave: 

  • Pressure cooker/ laboratory bench ( N type).  

  • Gravity displacement autoclave.

  •  Positive pressure displacement ( B type).

  • Negative pressure displacement (S type).

Types of Autoclave

Types of Autoclave

4. Where is the term autoclave taken from? 

In autoclave, auto comes from the Greek word and clave comes from the Latin word clevis which means self-locking device.

FAQs on Uses of Autoclave

1. How many types of autoclave machines do we have? What are those types?

There are three types of Autoclave machines.:

  1. Class N

  2. Class B

  3. Class S

2. Which bacteria is commonly used to check the efficiency of an autoclave machine?

Commercially, spores of Bacillus Stearothermophilus are used to check the efficiency of the autoclave machine.

3. What is the pressure in autoclave?

The pressure in the autoclave is about 15 psi or 103 k Pa.(1.02 atm.).

4. What is the temperature of the autoclave?

The temperature of the autoclave is approximately 121°c or 250°F.

5. How long does Autoclave sterilise?

Autoclave sterilises for 30 to 60 minutes.