Question

What kind of microscope uses acridine orange?
A. Phase contrast
B. Fluorescence
C. Transmission microscope
D. Scanning electron microscope

Answer 1

Hint: A microscope is an optical instrument used for viewing very small objects such as mineral samples, animal or plant cells, etc. It typically magnifies several hundred times so that the object viewed appears to be clear.

Complete answer:
a. Phase-contrast microscopy is a technique used for gaining contrast in a translucent specimen without staining the specimen. An advantage of the phase-contrast microscope is that it can be used with high-resolution objects, but it requires specialized condensers and expensive objects.
b. Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye. It has the ability to penetrate the cell membranes of acidic organelles of the sample.it also has ionic (cationic) properties which allow the dye to differentiate between various types of cells such as bacterial cells and white blood cells.it is used in an epifluorescence microscope.
c. Transmission electron microscopy (TEM) is a technique, in which a beam of electrons is transmitted through a specimen to form an image. Image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen.
A scanning electron microscope (SEM) scans a focused electron beam over a surface to create an image. The electrons in the beam interact with the sample and produce various signals that can be used to obtain information about the surface topography and composition of the sample of interest.

Note: The inventor of the microscope was Zacharias Janssen. But there are many theories about the invention of the microscope. Some historians say that Hans Lippershey was the first person who invented the microscope, and who was most famous for filing the first patent for a telescope at that time.