Question

In transgenics, the expression of transgene in target tissue is determined by A.Transgene
B.Promoter
C.Reporter
D.Enhancer

Answer 1

Hint: Transgene is a gene which has been transferred naturally or by other genetic engineering techniques from one organism to another.

Complete answer:
 A transgene is a gene which has been transferred naturally or through a few of a number of genetic engineering techniques from one organism to the other. The introduction of a transgene by a process known as transgenic and are likely to modify the phenotype of an organism. Transgene describes a part of DNA containing a gene progression that has been inaccessible from one organism and is introduced into a dissimilar organism. This non native segment of DNA may moreover hold the ability to create RNA or protein in the transgenic organism or vary the normal working of the transgenic organism’s genetic code. In common, the DNA is integrated into the organism’s germ line. In advanced vertebrates this can be accomplished by combining the foreign DNA into the nucleus of a fertilized ovum.

The building of a transgene needs the meeting of a few main parts. The transgene must contain a promoter, which is a regulatory pattern that will decide wherever and while the transgene is active, an exon, a protein coding sequence which is usually derived from the cDNA for the protein of interest, and a stop sequence. These are usually joint in a bacterial plasmid and the coding sequences are generally selected from transgenes with formerly known functions.

Transgenic or genetically modified organisms, may be bacteria, viruses or fungi, serve up many research purposes. Transgenic plants, insects, fish and mammals which also includes humans have been bred. Transgene get away has been documented for GMO crops from 2001 with determination and invasiveness. Transgenic organisms pretense ethical questions and may be reason biosafety problems
A reporter gene is a gene cloned with a DNA sequence of attention inserted into an expression vector which is followed by transfer to target tissues. Following transfer, the cells are assayed to notice the occurrence of the reporter by measuring the reporter protein or its enzymatic activity. A superior reporter gene is recognized easily and can be measured quantitatively when it is expressed in the tissue of interest. They are used as indicators to know the gene expression in target tissue for pharmaceutical and molecular biology research.

Hence, the correct answer is option (C)

Note: Reporter genes are genes which can be readily subsequent to transfection, and also are used as markers for screening transfected successfully.