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In the given methodology which has been used for cattle, sheep, buffaloes, etc., a few steps are marked as 1, 2, and 3. Identify 1, 2 & 3?
A) 1-FSH, 2-Superovulation due to induced follicular maturation, 3-Transfer to surrogate mother
B) 1-LH, 2-Superovulation due to induced follicular maturation, 3-Transfer to surrogate mother
C) 1-Progesterone, 2-Superovulation due to induced follicular maturation, 3-Transfer to surrogate mother
D) 1-Oestrogen, 2-Superovulation due to induced follicular maturation, 3-Transfer to surrogate mother

Answer
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Hint: The hormone which helps in follicular development and maturation is a complex process of interrelated intra-and extra ovarian events that ultimately lead to ovulation of a mature oocyte and the ruptured follicle changes into a corpus leuteum.

Complete step-by-step answer:
First of all, there is a methodology given which is used in cattle. a cow is given follicle-stimulating hormone. Rapid secretion of LH reaches its maximum level and induces rupture of graffian follicle and thereby release of an ovum(ovulation) so that means LH is not given. In step 2 superovulation is there due to induced follicular maturation which is not done by estrogen. And progesterone is also not given to cattle.
And FSH(follicle stimulating hormone) stimulates follicular development. Superovulation is a drug-induced production of multiple eggs for use in assisted reproductive technologies. Superovulation takes place due to induced follicular maturation and multiple eggs will be formed i.e., 6-8 eggs per cycle. After that, they were artificially inseminated and when they recovered then they were transferred to surrogate mothers. So it is the flow chart of superovulation. Such as in-vitro fertilization. Normally cattle from one to two eggs only per cycle and after copulation, it produces just one baby so by the use of superovulation multiple cattle can be produced.
Hence the correct answer is option A i.e., follicle-stimulating hormone.

Note: Superovulation in mice involves the administration of equine chorionic gonadotrophin to promote follicle growth and then that of human chorionic gonadotropin to induce ovulation.