Question

In anaerobic glycolysis 2 mol of inorganic phosphate are used per mole of glucose consumed. Which of the following enzymes catalyses the uptake of inorganic phosphate?
A. Hexokinase
B. Phosphofructokinase
C. Pyruvate kinase
D. Glyceraldehyde 3 phosphate dehydrogenase

Answer 1

Hint: Glycolysis is a process that involves the conversion of one molecule of glucose into two molecules of pyruvate, hydrogen ions, and two molecules of water. Glycolysis produces energy molecules like ATP, NADH. The pyruvate molecules produced during glycolysis will give rise to acetyl-CoA.

Complete answer:
The glycolysis process does not require the presence of oxygen hence it is an anaerobic process. The step involved in the glycolysis are as follows:
6 carbon compound glucose is converted into glucose 6- phosphate in the presence of enzyme Hexokinase. In this step, a molecule of ATP is used. The glucose 6-phosphate in the presence of phosphoglucose isomerase is converted into fructose-6-phosphate. Fructose 6-phosphate is then converted into fructose- 1,6- bisphosphate in the presence of phosphofructokinase. A phosphate group is transferred from ATP. Fructose- 1,6-bisphosphate is then split into 3 carbon sugars dihydroxyacetone phosphate and glyceraldehyde- 3-phosphate. They are isomers. But only one glyceraldehyde continues in the process of glycolysis.

Glyceraldehyde 3-phosphate is then converted into 1,3-diphosphoglycerate in the presence of glyceraldehyde 3-phosphate dehydrogenase. 2 molecules of NADH are produced at this step. 1,3- bisphosphoglycerate is then converted into 3-phosphoglycerate I. The presence of phosphoglycerate kinase. 2 molecules of ATP have been released in this step. 3-phosphoglycerate kinase is then converted into 2-phosphoglycerate in the presence of phosphoglycerate mutase.

2-phosphoglycerate is then converted into phosphoenolpyruvate in the presence of enolase. Two water molecules are released in this step. Phosphoenol pyruvate is then converted into pyruvate in the presence of pyruvate kinase where two molecules of ATP are released.

So, the correct answer is option A.

Note:
During the Glycolysis cycle, there are 4 ATPs and 2 NADH are produced. But 2 ATPs are used in the starting of the step of glycolysis, so 2 ATPs are produced at the end of glycolysis.