
E. coli is used in the production of
A) Rifampicin
B) LH
C) Ecdysone
D) Interferon
Answer
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Hint: E. coli is commonly used in the production of Enzymes, other proteins by Recombinant DNA technology. E. coli is mostly used in recombinants, because of its relative simplicity, inexpensive and fast high-density cultivation, and well-known genetics.
Complete answer:
E. coli is a Gram Negative, facultative, anaerobic, rod-shaped bacteria. It is a preferred host for gene cloning due to the high efficiency of introduction of DNA molecules into cells E. coli is a preferred host for protein production because of its rapid growth and the ability to express proteins at very high levels.
A) RIFAMPICIN: It is an antibiotic, used to treat a variety of serious infections. This is synthesized naturally by Bacterium Amycolatopsis rifamycinica. This antibiotic is used to treat or prevent Tuberculosis.
B) LH: Luteinizing Hormone is produced by pituitary glands released in the anterior pituitary gland. This hormone plays an important role in sexual development.
C) ECDYSONE: It is a steroidal hormone secreted by the prothoracic gland in insects. It plays an important role in stimulation of metamorphosis and regulates moulting in insects.
D) INTERFERON: Many proteins are produced by E. coli using recombinant DNA technology. Recombinant human interferon is produced and purified from E. coli transfected with the human interferon gene.
Thus, the correct answer is D) INTERFERON
Note:
Interferons are known as signalling proteins that are synthesized and released by host cells in response to the presence of infections. Interferon proteins are synthesized in host cells, encoded by the host genes in response to dsRNA. Through recombinant DNA technology Interferons are produced by E. coli.
Complete answer:
E. coli is a Gram Negative, facultative, anaerobic, rod-shaped bacteria. It is a preferred host for gene cloning due to the high efficiency of introduction of DNA molecules into cells E. coli is a preferred host for protein production because of its rapid growth and the ability to express proteins at very high levels.
A) RIFAMPICIN: It is an antibiotic, used to treat a variety of serious infections. This is synthesized naturally by Bacterium Amycolatopsis rifamycinica. This antibiotic is used to treat or prevent Tuberculosis.
B) LH: Luteinizing Hormone is produced by pituitary glands released in the anterior pituitary gland. This hormone plays an important role in sexual development.
C) ECDYSONE: It is a steroidal hormone secreted by the prothoracic gland in insects. It plays an important role in stimulation of metamorphosis and regulates moulting in insects.
D) INTERFERON: Many proteins are produced by E. coli using recombinant DNA technology. Recombinant human interferon is produced and purified from E. coli transfected with the human interferon gene.
Thus, the correct answer is D) INTERFERON
Note:
Interferons are known as signalling proteins that are synthesized and released by host cells in response to the presence of infections. Interferon proteins are synthesized in host cells, encoded by the host genes in response to dsRNA. Through recombinant DNA technology Interferons are produced by E. coli.
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