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During plasmolysis, water moves out from the cell, it is first lost from the
A. Cytoplasm and then from the vacuole
B. Vacuole and then from the cytoplasm
C. Cytoplasm and then from the nucleus
D. Nucleus and then from the cytoplasm

Answer
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Hint: Plasmolysis is a distinct response of plant cells subjected to hyperosmotic stress. Turgor losses cause the violent detachment of the living protoplast from the wall of the cell. The plasmolytic process is mostly led by the vacuole. Plasmolysis is reversible (deplasmolysis) and characteristic to living cells of plants.

Complete step-by-step answer:
Plasmolysis causes the shrinking of the protoplasm because of the loss of water from osmosis once a cell is in contact with a hypertonic solution.
If a cell contracts when placed in a solution, the solution is hypertonic because a hypertonic solution has a smaller concentration of solvent molecules than the fluid within the cell. So, the solvent molecules are forced outside the cell from the area of higher concentration to the lower concentration by the method of exosmosis. Plasmolysis takes place once water passes out of the cell and the protoplasm of the plant shrinks away from its cell wall. This takes place when the cell is positioned in a hypertonic solution (a solution that has more concentration of solute than the cytoplasm of the cell). Initially water has been lost from the cytoplasm and then from the vacuole. This causes the protoplast to shrink away from the walls, leading to the plasmolysis of the cell.
Therefore the correct answer is option A.

Note: Plasmolysis is the contraction of protoplasm away from the cell wall of a bacterium or a plant. The protoplasmic shrinking is often because of the loss of water through exosmosis, therefore which leads to gaps among the plasma membrane and the cell wall.