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An in situ method of conservation is
A. Botanical garden
B. National park
C. Genetic engineering
D. Cryopreservation
E. Tissues culture

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Answer
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Hint: In situ conservation techniques is the on-site conservation of genetic resources such as microbes, plants, and animals, and maintenance of genetic variations at the site where they come across, it may be a wild site or traditional farming.

Complete answer:
A well-maintained garden or parks that have many different species of plants, each plant tagged with their botanical name such garden is called a botanical garden. They have a collection of special types of plants, such as herb gardens, plants of different areas of the world. Some plants are placed in greenhouses like tropical plants, alpine plants, and exotic plants.
The parks are created by the national government to conserve natural resources and genetic resources; these parks are called National parks. Frequently, it preserves natural, semi-natural, or genetic variations. Every nation builds its own national parks in different areas to conserve wild nature and traditional farming systems.
Genetic engineering is also known as r-DNA technology or Gene cloning; it can be defined as changing genes by using in vitro processes. a gene of known function can be transferred from its Normal location into a cell (that of course, does not contain it) via suitable vectors. The transferred gene replicates normally and is handed over to the next progeny. On confirmation for its presence through biochemical procedures replica of the same cell or clone, can be produced the derivation of procedures for the reintroduction of the foreign gene fragment into a bacterium have led to evolution of new technology i.e. the recombinant DNA technology.
The process to preserve cells, tissue, organs, serum, germ cells in cooling is called cryopreservation. At the low temperature, the viability is maintained of living cells.
The rearing of cells or tissue in an artificial medium under controlled condition, such process is called tissue culture.
So, the correct answer is “Option B”.

Note:
Humans are directly or indirectly dependent on biodiversity for sustenance to a considerable extent. Hence, increasing population and developmental activities have led to large scale depletion of natural resources.