Question

Which one of the following is a recognition site for restriction enzyme Bam HI?
A. 5’ – GAATTUC – 3’3 – CTTAAG – 5’
B. 5’5 – GGATCC – 3’5 – CCATGG – 3’
C. 5’ – GGATCC – 3’3’ – CCTAGG – 5’
D. 5’ – GAATTC – 3’5’- CTTAAC – 3’

Answer 1

Hint: They cut by a nuclease and conceivably treating certain DNA change determined maladies through hereditary treatment for NARP and MILS conditions. For example, there are mitochondrial ailments that can be brought about by changes in the mitochondrial DNA.

Complete answer:
BamHI (from Bacillus amyloliquefaciens) is a sort II limitation endonuclease, having the limit to perceiving short groupings (6 bp) of DNA and explicitly dividing them at an objective site. BamHI ties at the acknowledgement succession 5'- GGATCC-3', and cuts these arrangements soon after the 5'- guanine on each strand. This cleavage brings about clingy ends which are four bp long. In its unbound structure, BamHI shows a focal b sheet, which lives in the middle of α-helices.
BamHI goes through a progression of conformational changes upon DNA acknowledgement. This permits the DNA to keep up its typical B-DNA compliance without contorting to encourage catalyst officials. BamHI is an asymmetric dimer. DNA is bound in an enormous parted that is shaped between dimers; the protein ties in a "hybrid" way.
Each BamHI subunit makes most of its spine contacts with the phosphates of a DNA half-site however base pair contacts are made between each BamHI subunit and nitrogenous bases in the significant depression of the contrary DNA half-site.

Hence, the correct answer is option (C).

Additional information:
Palindromic successions assume a significant part in atomic science. It is perceived by 5' – GAATTUC – 3'3 – CTTAAG – 5'.
SsoI is perceived by the site: 5' – GAATTC – 3'5'- CTTAAC – 3'.
The recognition site for restriction compound Srl32DII is 5'5 – GGATCC – 3'5 – CCATGG – 3'.

Note: The BamHI compound is equipped for making countless contacts with DNA. Water-interceded hydrogen holding, just as both primary chain and side-chain connections help in the official BamHI acknowledgement arrangement. In the significant depression, most chemical/DNA contacts occur at the amino end of the equal 4-helix pack, composed of a4 and a6 from every subunit. Although a6 from every subunit does not enter the DNA significant score, its previous circles collaborate with the external finishes of the acknowledgement site. BamHI ties at the recognition arrangement 5'- GGATCC-3'.