Question

Which of the following is required for the microinjection method of gene transfer?
A. Microparticles
B. Micropipettes
C. Divalent cations
D. UV radiations

Answer 1

Hint: Direct quality exchange incorporates move of the unfamiliar quality of enthusiasm into the host plant cell without the assistance of a vector. The microinjection method is one of the examples of direct gene transfer.

Complete Answer:
- We already know that transfer of genes is very much essential for various laboratory experiments and also plays a crucial role in genetic researches.
- The microinjection method is the most commonly used method applied by scientists to transfer genes. It is also known as vector less method because no vector is required to complete the method. In this process, a foreign DNA is injected directly into the nucleus of an animal as well as a plant cell.
- Generally, micropipettes or micro needles are used for the transfer. DNA microinjection is the ruling procedure prompting irregular reconciliation of a transgene through the presentation of DNA into the pronucleus of a creating zygote.
- Following the treatment of a mouse egg, the male and female pronuclei stay isolated for a couple of hours before they circuit to make the zygotic core, subsequently considering microinjection of the ideal qualities into the bigger male pronucleus.
- Eggs that can endure the infusions are moved into the oviducts of a temporary mother i.e., pseudopregnant female mice for the age of the Founder mouse, from which perpetual transgenic lines can be set up.
- The presence of transgenes in the offspring can be recognized by PCR (Polymerase Chain Reaction) investigation or Southern smudge hybridization.
- We need to learn that it is applicable during the transfer of DNA in the oocytes or eggs. Sometimes, the transfer of DNA in the embryo is also performed using a micropipette. Therefore, we can say that out of the above-mentioned options, micropipette is applicable.

Therefore, the correct answer is option ‘B’.

Note: Highlights of DNA Microinjection:
- The measure of DNA conveyed per cell isn't restricted and the conveyance is exact, the two of which increment the possibility for integrative change.
- As a direct physical methodology, this procedure has a range of autonomy.
- Cells that are infused with the DNA material have high recovery potential.
- Even though pronuclear microinjection is mainstream, this strategy for transgenesis has a few inconveniences, for example, low achievement rate and mosaic authors.