Question

Which blotting is used in DNA fingerprinting?

Answer 1

Hint: Blotting is a process that involves resolving a macromolecule like DNA, RNA, or protein in a gel matrix, then transferring it to a solid substrate and detecting it with a specific probe. These sophisticated tools enable researchers to locate and describe single molecules within a complicated mixture of related chemicals. Blotting is a technique used in molecular biology to identify proteins and nucleic acids, and it is commonly used in diagnostics.

Complete answer:
A blot is a method of transferring proteins, DNA, or RNA onto a carrier in molecular biology and genetics. In many cases, this is done after a gel electrophoresis, where the molecules are transferred from the gel to the blotting membrane, while in other cases, the samples are added directly to the membrane.
DNA fingerprinting, also known as DNA profile analysis, is based on analysing polymorphic sections of human DNA using the "Southern" hybridization or southern blotting technique. Southern blotting is a technique for detecting a specific DNA sequence in a blood or tissue sample in the laboratory. A restriction enzyme is used to break down a DNA sample into fragments that can then be separated using gel electrophoresis. The DNA fragments are transported from the gel to a membrane’s surface. The membrane is subjected to a radioactive or chemically tagged DNA probe. If the probe binds to the membrane, the sample contains the probe sequence.

So the correct answer is Southern blotting

Note:
Southern blotting transfer can be employed for homology-based cloning based on the amino acid sequence of the target gene's protein product. The sequence of oligonucleotides is designed to be comparable to that of the target sequence. Chemically produced, radiolabeled oligonucleotides are used to screen a DNA library or other collections of cloned DNA fragments. Sequences that hybridise with the hybridization probe are analysed further to acquire the full-length sequencing of the targeted gene, for example.