Question

Virus free plants can be obtained by
A) Antibiotic treatment
B) Bordeaux mixture
C) Root tip culture
D) Shoot tip culture

Answer 1

Hint: It is a culture of terminal that comprises Meristem together with the primordial and adjacent stem tissue and from the developing leaves. Virus free plants are those plants in which the test becomes negative for viruses.

Complete Answer:
Virus free plants can be obtained by shoot tip because the Meristem of the shoot is full of meristematic tissue that has immense potential to divide and damage cells are replaced quickly.
There are following steps for shoot tip culture given below:
- The shoot tip is cut with a knife that is sterilized and can be used as an explanation.
- Then the surface of the explant is sterilized. On a nutrient media the explant is cultured.
- Now the explant then turns into the callus. Callus is transferred to the media with high concentration of cytokinin for the shoot development.
- After that development it is then transferred to the routing media that contains a high concentration of auxin and low concentration of cytokinin.
- After growth of shoot and root it undergoes hardening treatment where it is grown under temperature available on fields to prepare them for growth.
- From the plant media the plant plates get removed and transferred to the soil.

Additional Information: Bordeaux mixture is a combination lime, copper sulphate and water and an effective bactericide that is used to control diseases. Root tip cultures are generally used in the liquid medium and culturing of radical tips of seed germination.

The correct answer is option C i.e., shoot tip culture.

Note: Virus free plants can be obtained by the help of meristem technique or culture. Other pathogens like fungi and bacteria also get eliminated by this shoot tip culture. Virus free plants are those in which the test for virus becomes negative.