Question

The southern blot technique is utilized for the detection of
(A) DNA
(B) RNA
(C) Protein
(D) DNA and RNA

Answer 1

Hint: Southern blotting was taught by Edwin Southern in 1975 as a technique to observe specific sequences of DNA in DNA samples. The RNA samples can be segregated on agarose gel with formaldehyde as the denaturing agent that restricts the secondary structures of RNA molecules.

Complete answer: -Restriction endonucleases are utilized to cut high-molecular-weight DNA strands into smaller fragments.
-The DNA fragments are then electrophoresed on an agarose gel to distinguish them by size.
-The DNA components are bigger than 15 kb before blotting, the gel may be dealt with an acid, such as dilute HCl. Alkaline transfer procedures are utilized, the DNA gel is placed into an alkaline solution to denature the double-stranded DNA.
-A sheet of nylon membrane is positioned on top of or below, depending on the way of the transfer of the gel. The pressure is assigned evenly to the gel to assure good and even reference between gel and membrane.
-The membrane is then baked in a vacuum or regular oven at 80 degree celsius for 2 hours.
-The membrane is then disclosed to a hybridization probe. After hybridization, the excess probe is washed from the membrane.
Thus, the correct option among all of these is Option A - DNA

Note: Southern blotting is a laboratory procedure used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to slice a sample of DNA into components that are distinguished using gel electrophoresis.