
The experimental proof for semiconservative replication of DNA was first shown in
A. Plant
B. Fungus
C. Virus
D. Bacterium
Answer
572.1k+ views
Hint: DNA replication is semiconservative and this was first proven experimentally by Meselson and Stahl on E.coli. Later many more experiments were conducted by the different scientists on different organisms.
Step by step answer:Semi conservative mode of DNA replication was first proposed by Watson and Crick. And then it was proved by Meselson and Stahl(1958) while working on bacteria E.coli and also by Taylor while working on plant Vicia faba(1958). To prove this, Taylor used a radioactive technique in which radioisotopes(tritiated thymidine) were used.
Meselson and Stahl used a heavy isotope of nitrogen and Cairns(1963) used radioactive thymidine.
Meselson and Stahl grew many generations of bacteria in a medium having a heavy isotope of nitrogen. This labeled DNA was transferred to a normal medium( ie. having light nitrogen). The generation obtained was hybrid, the second generation has 50% hybrid and 50% light type DNA. This result is possible only if at each replication one strand is conserved in the daughter while the second is freshly synthesized.
As the first experiment to prove the semiconservative nature of DNA replicated was done on E.coli, which is a bacteria, so the correct option is D.Bacterium
Note: DNA replication is a bidirectional process that occurs during the S-phase of the cell cycle.DNA replication occurs in all living organisms. DNA is the only molecule capable of self-replication or duplication, so it is also termed as a living molecule.
Step by step answer:Semi conservative mode of DNA replication was first proposed by Watson and Crick. And then it was proved by Meselson and Stahl(1958) while working on bacteria E.coli and also by Taylor while working on plant Vicia faba(1958). To prove this, Taylor used a radioactive technique in which radioisotopes(tritiated thymidine) were used.
Meselson and Stahl used a heavy isotope of nitrogen and Cairns(1963) used radioactive thymidine.
Meselson and Stahl grew many generations of bacteria in a medium having a heavy isotope of nitrogen. This labeled DNA was transferred to a normal medium( ie. having light nitrogen). The generation obtained was hybrid, the second generation has 50% hybrid and 50% light type DNA. This result is possible only if at each replication one strand is conserved in the daughter while the second is freshly synthesized.
As the first experiment to prove the semiconservative nature of DNA replicated was done on E.coli, which is a bacteria, so the correct option is D.Bacterium
Note: DNA replication is a bidirectional process that occurs during the S-phase of the cell cycle.DNA replication occurs in all living organisms. DNA is the only molecule capable of self-replication or duplication, so it is also termed as a living molecule.
Recently Updated Pages
A man running at a speed 5 ms is viewed in the side class 12 physics CBSE

State and explain Hardy Weinbergs Principle class 12 biology CBSE

Which of the following statements is wrong a Amnion class 12 biology CBSE

Two Planoconcave lenses 1 and 2 of glass of refractive class 12 physics CBSE

The compound 2 methyl 2 butene on reaction with NaIO4 class 12 chemistry CBSE

Bacterial cell wall is made up of A Cellulose B Hemicellulose class 12 biology CBSE

Trending doubts
What are the major means of transport Explain each class 12 social science CBSE

Which are the Top 10 Largest Countries of the World?

Draw a labelled sketch of the human eye class 12 physics CBSE

Explain sex determination in humans with line diag class 12 biology CBSE

The pH of the pancreatic juice is A 64 B 86 C 120 D class 12 biology CBSE

Give 10 examples of unisexual and bisexual flowers

