Question

The DNA molecule into which the gene of interest is integrated for cloning is called as
A. Carrier
B. Transformer
C. Vector
D. Template

Answer 1

Hint: Molecular cloning for the most part utilizes DNA groupings from two distinct living beings: the species that is the DNA source that has to be cloned, and the species that will act as the living host for replication of the recombinant DNA.

Complete answer:
In a conventional molecular cloning test, the DNA to be cloned is acquired from a living being of intrigue, at that point treated with proteins in the test cylinder to create littler DNA pieces. Thus, these sections are then joined with vector DNA to produce recombinant DNA atoms. The recombinant DNA is then brought into a host creature (normally a simple to-develop, considerate, lab strain of E. coli microorganisms). This will create an organism population wherein recombinant DNA particles are repeated alongside the host DNA. Since they contain unfamiliar DNA parts, these are transgenic or hereditarily altered microorganisms (GMO). This measure exploits the way that a solitary bacterial cell can be initiated to take up and repeat a solitary recombinant DNA particle.

In standard molecular cloning tests, the cloning of any DNA piece basically includes seven stages:
(1) Choice of host living being and cloning vector,
(2) Preparation of vector DNA,
(3) Preparation of DNA to be cloned,
(4) Creation of recombinant DNA,
(5) Introduction of recombinant DNA into have life form,
(6) Selection of creatures containing recombinant DNA,
(7) Screening for clones with wanted DNA embeds and organic properties.

Hence, the correct answer is option (C).

Additional information:
Plasmids are the self-replicating circular DNA molecules in the bacterial cells. They are used for transferring desired genes into the other cells which are known as vectors.

Note: Molecular cloning furnishes researchers with a basically boundless amount of any individual DNA fragments obtained from any genome. This material can be utilized for a wide scope of purposes, incorporating those in both fundamental and applied organic science.