Question

The correct sequence in the tissues culture technique is
a. Inoculation – explain incubation – callus formation- organogenesis – preparation of medium – transferred to the field
b. Preparation of medium- inoculation- explant incubation- callus formation - organogenesis- transferred to field
c. Explain incubation- preparation of medium- inoculation- organogenesis- callus formation- transferred to field
d. Preparation of medium- inoculation- organogenesis- callus formation- transferred to the field

Answer 1

Hint: Tissue culture technique is a technique in which cells are grown artificially in the laboratory. Tissue culture in plants is the process by which small fragments of a plant are introduced into an artificial nutrient medium, it allows its growth and functioning. In short, plant fragments or cells are placed in an artificial growth medium to grow into a new plant.

Complete answer:
- Many steps are involved in the tissue culture technique. In tissue culture, the first step is the preparation of the medium.
- For the preparation of medium, a growth medium in the form of agar or broth is used containing appropriate nutrients. The selected mother plant is inoculated in the medium.
- Once the inoculation is done, incubation of the explant occurs under proper condition of temperature and under a sterilized environment.
- On the medium, the unorganized tissue mass grows called Callus. The process of organogenesis has two distinct phases, dedifferentiation and redifferentiation.
- The dedifferentiation process starts after the isolation of the explant. The process of redifferentiation begins after the first callus cell forms followed by organogenesis.
- Then, the plant is transferred to the field.

Hence, the correct answer is option (B).

Additional information:
- Plant tissue culture technique is used in forestry, plant sciences, and in horticulture.
- Plants produced commercially are used as landscape, potting, and florist subjects, that use shoot and meristem culture to produce large numbers of identical individuals.
- New plants produced are disease-free.
- A mature plant is produced quickly by tissue culture.
- Tissue culture needs more cost and labor.

Note: The tissue culture technique is the process in which cells are grown in the artificial medium. There are various methods used for tissue culture. By this technique, we can produce plants with desirable traits. The tissue culture technique plays an important role in biological studies. The commercially important number of hybrids are routinely propagated by tissue culture technique. It is concluded that the tissue culture technique is of great importance to plant sciences, forestry, and horticulture.