Question

Southern blotting Technique uses -----paper for embedding DNA strands.
(a)Whatman’s No:1
(b)Parchment
(c)Nitrocellulose
(d)Cellophane

Answer 1

Hint: This is a mild explosive, used in rockets, propellants, printing ink bases, leather finishing, and celluloid. This is also known as Guncotton. This is a compound produced by the reaction of cellulose and nitric acid, or other strong nitrating agents.

Complete answer:
Southern blotting is used to detect specific DNA sequences from the given sample of DNA.
-The first step in a Southern blot is to prepare the DNA mixture by breaking them into small fragments of separate DNA’s using a restriction enzyme.
-The mixture of DNA fragments is separated according to the size by gel electrophoresis.
-Then, the double-stranded pieces of DNA are denatured into single strands by the use of gel.
-Next, the DNA is transferred from the gel into a blotting membrane which is known as the nitrocellulose membrane.
-The DNA is labeled using radioactive probes which can be then detected by autoradiography.

Additional Information: The advantage of Southern blotting offers genetic testing is that it can be used to detect DNA identity, size, and abundance. If a scientist wants to look at whether an organism has a particular gene mutation, southern blotting can be used to identify the presence of that mutated gene.
Procedure/ Steps:
-Restriction digest: done by using RE enzyme and amplification by PCR
-Gel electrophoresis: through SDS gel electrophoresis
-Denaturation: It is done by treating with HCl and NaOH
-Blotting-Nitrocellulose
-Baking and Blocking by using casein in BSA
-Hybridization done with the help of labeled probes
-Visualization by the use of an autoradiogram.
So, the correct answer is, ‘Nitrocellulose.’

Note: DNA fragments from a gel are transferred to a nitrocellulose paper during the process, Southern blotting. The main aim of transferring the DNA from a gel to a nitrocellulose paper is to:
- To attach the DNA fragments to a substrate permanently.
- Separate the 2 complementary DNA strands.