Question

Some of the steps involved in the production of humulin are given. Choose the correct sequence
(i) Synthesis of a gene (DNA) for human insulin artificially (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (v) Introduction of the recombinant plasmid into E. coli (vi) Extraction of a recombinant gene product from E. coli.

A. (ii), (i), (iv), (iii) (v), (vi)
B. (i), (iii), (v), (vi), (ii), (iv)
C. (i), (iv), (v), (ii), (vi), (iii)
D. (iii), (v), (ii), (i), (vi), (iv)

Answer 1

Hint: Insulin is a peptide hormone that promotes the absorption of glucose into the liver from the blood. Insulin medicine is given to the diabetic person suffering from diabetes mellitus 1 and 2. As insulin’s production from the human and animal body became difficult, the recombinant technology made it easier to synthesize the humulin, an alternative.

Complete answer:
Humulin is an amorphous, crystalline suspension of human insulin with zinc as intermediate insulin with a slow rate of inset and with a longer duration of activity than normal insulin. It is produced by recombinant technology using E.coli. People use it as it is a short-acting insulin. It helps to regulate the blood sugar level to gain energy.

The procedure of production is humulin is as follows:-
1. Synthesis of the gene (DNA) for human insulin artificially.
2. Insertion of the human insulin gene into the plasmid.
3. Introduction of the recombinant plasmid into E.coli
4. Culturing recombinant E.coli in bioreactors.
5. Extraction of a recombinant gene product from E.coli.
6. Purification of Humulin.

So the answer is option C: (i), (iv), (v), (ii), (vi), (iii)

Note: Humulin N is isophane insulin given to a diabetic patient to control the blood sugar level. It is given twice a day via injection. Its effects last for 12-18 hours. Humulin R is short term insulin which lasts for 8 hours. There are various side effects like weight gain, skin rash, swelling on feet etc. insulin was invented by Sir Fredrick B Banting, Charles H Best and JJR Macleod in 1922. El Lily invented humulin 1982 in the U.S.A.