Question

Select the correct options to fill up the blanks.
(i) _____ is a natural polymer extracted from ______.
(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.
(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.
(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______.
(v) Through _____ in agarose gel electrophoresis.

A. (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size (v) sieving effect
B. (i) Agarose, seaweeds (ii) restriction site, antibiotic resistance genes (iii)recombinant DNA, cloning vector (iv) Taq polymerase, size (v) sieving effect
C. (i) Agarose, seaweeds (ii) restriction site, antibiotic resistance genes (iii) A-DNA, cloning vector (iv) Taq polymerase (v) Size, sieving effect
D. (i) Size, sieving effect (ii) Agarose, seaweeds (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Answer 1

Hint: Polymers are those materials made up of long, repeating chains of molecules. In agarose gel electrophoresis uses such a polymer layer to separate nucleic acids.

Complete Answer:
Let us find out the answer to the blanks.
(i) Agarose is a natural polymer extracted from sea weeds.
(ii) The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with the cloning vector.
(iii) The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector.
(iv) Taq polymerase enzyme remains active during the high temperature-induced denaturation of dsDNA. DNA fragments are resolved according to their size.
(v) Through a sieving effect in agarose gel electrophoresis.

So the answer to the blank: (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size (v) sieving effect

Option A: Since the above mentioned answer matches this option i.e., (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size (v) sieving effect. This is the correct option.


Option B, C & D: Since the correct answer to the blank does not match with these options. Therefore, this is the incorrect option.

Thus, the correct option is A) (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size (v) sieving effect.

Note: Agarose is used for agarose gel electrophoresis which is a process for the separation of bands of nucleic acids for their identification. Taq polymerase is obtained from Thermus aquaticus.