
Production of hybridoma is an example of
A. Transgenesis
B. Cell cloning
C. Gene cloning
D. None of the above
Answer
565.5k+ views
Hint: Hybridoma technology is a technique for producing great numbers of identical antibodies. This process begins by injecting a mouse with an antigen that inflames an immune response. A type of white blood cell, the B cell, creates antibodies that attach to the injected antigen.
Complete answer:
Hybridoma technology is a technology of producing hybrid cell lines (known as hybridomas) by joining an antibody-producing B cell through a myeloma (B cell cancer) cell that is picked for its capacity to produce in tissue culture and for an absence of antibody chain synthesis. The hybrid cell, which is hence produced, can be cloned to create many similar daughter clones. These daughter clones then produce the immune cell product. While these antibodies come from only one type of cell (the hybridoma cell) they are known as monoclonal antibodies.
Transgenesis is the course of introducing a gene from one organism into the genome of another organism. The plan is that the resulting transgenic organism will express the gene and display some new property or feature.
Gene cloning is the course in which a gene of interest is situated and cloned out of DNA extracted from an organism. When DNA is pulling out from an organism, all of its genes are extracted at one time.
Hence, the correct answer is option (B).
Note: The hybridomas can be raised in the culture, each culture initiating with one viable hybridoma cell, producing cultures each of which is composed of genetically alike hybridomas which create one antibody per culture (monoclonal) rather than mixtures of diverse antibodies (polyclonal). The myeloma cell line that is used in this process is selected for its capability to grow in tissue culture and for a nonappearance of antibody synthesis.
Complete answer:
Hybridoma technology is a technology of producing hybrid cell lines (known as hybridomas) by joining an antibody-producing B cell through a myeloma (B cell cancer) cell that is picked for its capacity to produce in tissue culture and for an absence of antibody chain synthesis. The hybrid cell, which is hence produced, can be cloned to create many similar daughter clones. These daughter clones then produce the immune cell product. While these antibodies come from only one type of cell (the hybridoma cell) they are known as monoclonal antibodies.
Transgenesis is the course of introducing a gene from one organism into the genome of another organism. The plan is that the resulting transgenic organism will express the gene and display some new property or feature.
Gene cloning is the course in which a gene of interest is situated and cloned out of DNA extracted from an organism. When DNA is pulling out from an organism, all of its genes are extracted at one time.
Hence, the correct answer is option (B).
Note: The hybridomas can be raised in the culture, each culture initiating with one viable hybridoma cell, producing cultures each of which is composed of genetically alike hybridomas which create one antibody per culture (monoclonal) rather than mixtures of diverse antibodies (polyclonal). The myeloma cell line that is used in this process is selected for its capability to grow in tissue culture and for a nonappearance of antibody synthesis.
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