Question

What is plant tissue culture? What are the important steps involved in it?

Answer 1

Hint:Plant tissue culture is a technique in which the new plant can grow from a cell in a test tube inside a laboratory. This capacity of a plant to regenerate into a new plant is called totipotency.

Complete answer:
To solve this question, we must know what tissue culture is.
Plant tissue culture is the technique used to grow plant cells, tissues or organs under sterile conditions on a nutrient culture media. It is widely used to produce clones. Cells, plant protoplasts, pieces of leaves, stem or roots can be used to grow a new plant on a culture media which is enriched with growth hormones and nutrients.
Steps Involved:-
>This method is done in a sterile condition using a laminar air flow which uses HEPA filters for making the surrounding sterile and the tissue is grown on a sterile container.
>The sterile explanation is grown on a sterile nutrient media which is rich in nutrients and hormones.
>The cell or tissue grows as callus. It is then grown as small plantlets.
>These plantlets are transferred to the field or greenhouse to grow as natural plants.

Note:The advantages of plant tissue culture are:-
->It quickly produces mature plants.
->The exact copies of the plants having desirable traits can be produced.
->multiple copies of plants can be produced in the absence of seeds or pollinators.
->The use of sterile containers reduces the chances of transmitting disease or pathogens.