Question

Okazaki fragments are joined in a correct sequence by
A) DNA enzyme I
B) DNA ligase
C) Polymer enzyme
D) Primase
E) Helicase

Answer 1

Hint: Okazaki fragments are short sequences of DNA (approximately one hundred fifty to two hundred base pairs long in eukaryotes) that are synthesized discontinuously and later joined along by the enzyme DNA ligase to make the continuous strand throughout DNA replication.

Complete answer:
Helicases move on the double-stranded DNA and separate the strands by breaking H- bonds using ATP as an energy source. DNA ligase enzyme joins 2 Okazaki fragments via phosphodiester bond between 3' end at the tip of 1 fragment and a 5' phosphate at the tip of another fragment. DNA enzymes serve clean-up functions throughout replication, recombination, and repair. It's 3' to 5' proofreading nuclease activity and 5' to 3' nuclease activity.
The 5' to 3' nuclease activity of DNA enzyme I will replace miscalculation containing the section of DNA/RNA, paired to the example strand that is then at the same time replaced by enzyme activity of an equivalent enzyme. Primase synthesizes RNA/DNA primer that could be a stranded section (complementary to the template) and features a free 3’ -hydroxyl cluster to facilitate the addition of nucleotides by enzyme enzymes.
A polymer enzyme synthesizes polymer mistreatment DNA example strands by adding the ribonucleotides to a primer in 5' to 3' direction via phosphodiester bonds.

Hence, the option B is the correct answer.

Note:
DNA ligase could be a specific variety of enzyme, a ligase, that facilitates the formation of a phosphodiester bond between nucleotides. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, however some forms could specifically repair double-strand breaks.