Question

Name the source organism that possesses Taq polymerase. What is so special about the function of this enzyme?

Answer 1

Hint: Bacterium Thermus aquaticus is a substance in which the Taq polymerase obtained. The activation always remains during the high temperature that’s induced in the denaturation of DNA nucleotides in the PCR reaction with the help of thermostable DNA polymerase enzyme.

Complete answer:
Taq polymerase is a thermostable enzyme having molecular weight 94 KDa that’s used during the polymerase chain reaction introduced by Kary Mullins in the year 1983. First time it is isolated from the thermophilic bacteria that inhabit hot springs and the most significant feature of Taq polymerase is that enzyme is active at very high temperature during the polymerase chain reaction.

The various types are present inside the Taq polymerase such as specificity, yield and selectivity are the non-hot start taq polymerase and antibody based hot start taq, chemically modified hot start taq, sequester primers and wax bead based hot start taq are the types of hot start taq polymerase.

During the DNA replication, DNA makes new strands of DNA, using the existing strands as templates by using the DNA polymerase enzyme also known as Taq polymerase. It is a thermostable DNA polymerase enzyme and active at a high temperature during the process that occurs in the polymerase chain reaction.

Note: The short segment of the DNA quantity amplified by using the polymerase chain reaction (PCR). The enzyme used in this is able to withstand the protein denaturation condition at the high temperature that’s used during the thermostable State in the PCR.