Question

Mention four features of pBR322.

Answer 1

Hint:One of the first genetically modified plasmids is pBR322. It includes the genes for ampicillin and tetracycline resistance and it can be amplified by PCR.

Complete answer:
First we should know about plasmid to answer this question. Plasmids are molecules of extrachromosomal DNA. They are circular, small, and have an autonomous capacity to replicate. Plasmid replication is not under the influence of chromosomal DNA. In bacteria, they are often contained. Their potential to replicate independently allows plasmid a cloning vector to transfer and modify genes in the recombinant DNA technology. To pass genes from one organism to another, plasmids are used to produce recombinant DNA with the desired gene. This is regarded as genetic engineering. One of the first multipurpose EK2 cloning vectors to be developed and established (ten years ago) for the successful cloning and selection of recombinant DNA molecules in Escherichia coli was the plasmid pBR322.

The four features of pBR322 are:
>Sites of restriction: Hind III, BamHI, Pvu I, Sal I, Pst I, Pvu II, Cla I, EcoR I.
>Selectable marker: genes for ampicillin (ampR) and tetracycline (tetR) antibiotic resistance
>ORI: the origins of replication
>ROP: Codes for proteins that are active in the plasmid replication process.

Note:The cloning vector of pBR322 has the elements, that are the plasmid p, B is the Bolivar (the scientist's name), R is the Rodriguez (the scientist's name), and 322 represents the number of plasmids contained in the same laboratory.