Question

Match column-I with column-II and select the correct answer using the codes given below.

Column-I	Column-II
A. Southern blotting	I. Running of DNA fragments on get.
B. Gel electrophoresis	II. Cutting of DNA into fragments.
C. Cleavage	III. Transfer of DNA
D. DNA probing	IV. Searching for desired DNA fragments

A. A-IV; B-I; C-II; D-III
B. A-III; B-I; C-II; D-IV
C. A-IV; B-I; C-III; D-II
D. A-I; B-IV; C-II; D-III

Answer 1

Hint: Gel electrophoresis is a method used to isolate the DNA fragments (or other macromolecules, like proteins and RNA) depending on their size and charge. Southern blotting describes a procedure to identify the specific sequences of DNA. Double-stranded DNA can be cleaved at a particular site using restriction enzymes. A probe is a single-stranded sequence of RNA or DNA used to find its complementary sequence in a sample genome.

Complete Step-by-step answer:

A Southern blot is a technique used in molecular biology for the detection of a particular DNA sequence in DNA samples. Southern blotting combines the transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection through probe hybridization. Gel electrophoresis is the lab method used to divide mixtures of RNA, DNA, or proteins in accordance with molecular size. In gel electrophoresis, the molecules to be separated are pushed through an electrical field through a gel containing small pores. Cleavage defines the cutting of DNA to get DNA fragments. DNA fragments cut by restriction enzymes are referred to as restriction fragments. DNA probing is a technique to identify a segment of DNA, using a known sequence of nucleotide bases from a DNA strand to locate a complementary sequence in the sample with the help of base pairing.

Therefore the correct answer is Option B.
Note: Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. Gel electrophoresis is being used for a range of purposes, like obtaining DNA fingerprints for forensic purposes, checking a PCR reaction, and paternity testing. DNA probes are stretches of single-stranded DNA used for the detection of complementary nucleic acid sequences through hybridization. The DNA cleavage occurs away from the recognition location: more than 400 bp away to as far as 7000 bp.