Question

${K_m}$ value is related to
A. Chromatography
B. ES complex
C. ABO complex
D. Morphometry

Answer 1

Hint: Various reactions are occurring in our body. The enzymes react with the substrate via lock and key theory to give the possible products. Various methodologies are performed in the lab to know the components of the organic matter. Everything occurring in our body system has a physical attribute to it.

Complete answer:
${K_m}$ value or Michaelis constant is defined as the substrate concentration at which the half of the enzyme is forming ES complex concentration of substrate when the velocity of the enzyme reaction is half the maximum value.

> Option A: Chromatography is the technique of knowing the components in an organic matter in a stationary and mobile phase. They give the bands whose distance is measured and calculated via the Rf value.

> Option B: ES complex is formed when the enzyme and substrate bind to each other to make the product. This is the intermediate peak point when the ES complex breaks to form the product. The value is measured in the ${K_m}$ value as it demonstrates the substrate concentration when the velocity of the reaction is half the maximum value.

> Option C: ABO complex is in the blood groups. The antigens and antibodies on the RBC surface form the complex and give us the blood group.

> Option D: Morphometry is the process of measuring the external shape and dimensions of landforms and living organisms.

Hence, the correct answer is option (B).

Note: The${K_m}$ varies from enzyme to enzyme. Its tendency to combine with the substrate is different for each enzyme. It is used for allocating the different enzymes. A smaller ${K_m}$ value depicts high affinity of the enzyme to the substrate and the higher ${K_m}$ shows the less affinity.