Question

In the treatment of SCID, cDNA is introduced into a patient's cells using.............as a vector.

A. E Coli

B. Retrovirus

C. Bacillus thuringiensis

D. Agrobacterium

Answer 1

Hint: cDNA is to be synthesized, from an mRNA or messenger RNA template. It is synthesized in a reaction that is catalyzed via the use of reverse transcriptase and DNA polymerase enzymes. Essential to study is that cDNA is usually used to clone eukaryotic genes in prokaryotes.

Complete- Step-by Step-Answer:

Severe combined immunodeficiency (SCID) is a team of rare issues precipitated by using mutations in distinct genes concerned with the development and function of infection-fighting immune cells. Infants with SCID appear healthful at birth however are surprisingly inclined to extreme infections. The situation is fatal, generally within the first 12 months or two of life, until toddlers obtain immune-restoring treatments, such as transplants of blood-forming stem cells, gene therapy, or enzyme therapy. More than 80 per cent of SCID children no longer have a household history of the condition. However, the development of a new child screening takes a look at has made it viable to detect SCID earlier than signs appear, helping ensure that affected infants acquire life-saving treatments.

A good ADA (cDNA) can be delivered into the cells of the sufferers receiving gene treatment by using a vector constituted with the resource of retrovirus. The SCID-affected individual has an inaccurate gene for the enzyme Adenosine Deaminase (ADA). He/she lacks beneficial T-lymphocytes which are extracted from the patient's bone marrow and a day-to-day useful reproduction of human gene coding for ADA is delivered into these lymphocytes with the assistance of retrovirus.

Hence, the correct option is (B) Retrovirus.

Note: A retrovirus is a type of virus that inserts a DNA copy of its RNA genome into the DNA of a host mobile phone that it invades, for that reason changing the genome of that mobile phone.