Question

In RNAi, Genes are silenced using
a. ssDNA
b. dsDNA
c. dsRNA
d. ssRNA

Answer 1

Hint: RNA interference is the process of silencing protein translation. It helps in the regulation of protein synthesis. Translation involves synthesis of proteins from RNA . Double stranded RNA contains two complementary sequences which reduces the gene expression.

Complete answer:
The regulation of gene expression in a cell to prevent the expression of certain genes is called gene silencing. It can occur during transcription or translation. The silenced gene does not show any expression.

RNA interference is the biological process that occurs in all eukaryotic opposites. It involves silencing of mRNA due to a complementary dsRNA molecule. This double stranded molecule binds to and prevents translation of the mRNA (silencing). The RNA induced silencing complex (RISC) controls the RNA-dependent gene silencing process. The short double-stranded RNA molecule starts this process in a cell’s cytoplasm. They interact with the catalytic RISC component argonaute.

The process of replication occurs through RNA intermediate in some of the viruses or the mobile genetic elements (transposons) which contain the complementary RNA. The targeted mRNA degradation occurs when the sequence specific dsRNA is introduced into cells.
From the above information we have found that in RNAi, Genes are silenced using dsRNA.

Hence, the correct answer is option (C).

Note: RNA interference is very useful for the treatment of diseases. It can be used to silence specific genes linked to disease. The RNA is an unstable molecule so it is easier to manipulate it. It encodes DNA into protein sequences and performs critical biological functions. RNAi provides immunity against foreign viruses, regulation of genes and studying gene functions.