Question

In living cells, mitochondria can be stained with
A. Neutral red
B. Crystal violet
C. Aceto-orcein
D. Acid fuchsin

Answer 1

Hint: Mitochondria is the subcellular organelle, double membrane bounded, semi-autonomous, which is present in the cell, and it is the powerhouse of the cell because it produces energy by the kreb cycle. Mitochondria possess their own hereditary material (DNA) which help in formation of its own copy.

Complete answer:
Mitochondria is a subcellular cell which shows endo-symbiotic relationship evolution. In some cases, some cells may lack mitochondria, for example in red blood cells. Mitochondria generates ATP energy molecules. Mitochondria consist of outer and inner membranes and in between two membranes, the intermembrane space is present, cristae and matrix are present along with DNA.
Mitochondria organelle is stained by acid fuchsin dye by altmann’s method which consists of picric acid, aniline and acid fuchsin.

Hence, the correct answer is option (D).

Additional information:
> Option A: Neutral red-
This dye stains the lysosomes, this neutral dye acts as a pH indicator and changes from red to yellow in pH between 6.8 to 8.0. Lysosomes act as a suicidal bag of the cell, which arises from Golgi apparatus. This cell contains various enzymes such as hydrolytic enzymes which destroy the defected cell or waste product of the cell.

> Option B: Crystal violet-
It is methyl violet dye which is used in histology to stain the gram bacteria for classifying the bacteria. It is not only to stain bacteria but also used as textile dye and it shows antifungal and antibacterial properties.

> Option C: Aceto-orcein-
It is a stain which consists of orcein with acetic acid in solution and it is used to stain nucleic acid.

Note: Various kinds of dye are used to study the cell's example methylene blue which is used to stain bacteria for observation under a microscope. Different dye stains different cell organelle and their parts which form complex structures and highlight the stained part of the cell.