Question

Expand ‘YAC’ and mention what it was used for.

Answer 1

Hint: ‘YAC’ stands for yeast artificial chromosomes. It is used for mapping and sequencing of genomes. When yeast cells are multiplied, at that point they can be isolated and put into work with DNA mapping and sequencing.

Complete answer:
- It is a process which undergoes the process of replication.
- It works the same way as in plasmids and cosmids.
- In experiment with YAC, there are certain types of elements which are required for its correct results.
- Centromeres and telomeres replicate origin for YAC, before DNA cloned, they should be added in it.
- Yeast Spheroplast’s transformation can be construed.
- YAC’s are not circular in context to vectors, but they definitely consist of linear DNA.
- Genetic materials are added into YAC’s through large segments.
- YAC’s are having a perk over BAC’s as they can be used to show eukaryotic protein which needs post translational modification.
- It can also use clones to assemble all the genomes of organisms.
- The Average amount of DNA which can be cloned varies from 200 to 500 kb.
- Whereas, 1 MB can easily be cloned in YAC.
- Long research cannot be carried out on YAC because of its complicated structure.
- The process of cloning is less efficient in YAC.
- For the purpose of entire genomes, YAC’s are very popular.
- They are used to study chromosome behaviour.

Note: In the cloning system, YAC’s provide the largest insert. In YAC, the sequences remain more stable, represented, and intact. They can be reintroduced in mammalian cells and genes.