Question

Entamoeba histolytica can be cultured in
A. CLED medium
B. Maconke agar
C. Diamonds medium
D. NNN medium

Answer 1

Hint:
It was tested in a clinical context for the isolation and identification of bacteria and yeasts from the skin and ears of dogs using cystine, lactose, electrolyte-deficient (CLED) medium. A culture medium, used for the isolation and identification of bacteria from the human urinary system. Cystine and lactose are present, and it lacks electrolytes, which prevents Proteus organisms from swarming.
The pH will be lowered and the medium's colour will change from green to yellow in the presence of lactose-fermenting organisms. The pH indicator Bromthymol Blue's presence indicates the colour change. The substance that solidifies the mixture is agar. All potential urinary pathogens as well as a number of pollutants, including diphtheroid, lactobacilli, and micrococci, are encouraged to thrive. This media appeals to Candida as well.

Complete answer:
Entamoeba histolytica trophozoites grew well when casein hydrolysate was used in place of trypticase in Diamond's axenic culture medium TPS-1. Trophozoites that had been frozen in liquid nitrogen for 16 months were also able to thrive on this modified media. Microscopic analysis of faecal preparations for distinctive cysts and/or trophozoites can be used to identify Entamoeba histolytica. On the basis of the cysts' appearance and size, E. histolytica cysts are distinguished from those of nonpathogenic amoebic species.
So, Entamoeba histolytica can be cultured in Diamond medium.

Option ‘C’ is correct

Note:
Gram-negative organisms that are not picky can be grown on MacConkey Agar. As a result, MacConkey needed a method to restrict this background of environmental flora and permit solely the growth of his target organisms. Selective media is now a term used to describe a medium that can serve this purpose.
It is used to distinguish gram-negative bacteria that digest lactose from those that do not. It is employed for the isolation of intestinal pathogens and coliforms from water, dairy products, and biological samples.