
Differentiate between transformation and transduction.
Answer
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Hint: Transformation and transduction are the two main processes that can be used to transfer DNA matter from one bacterial cell to another. Transduction is considered as a tool that is used to insert the gene of interest into a bacterial cell or host’s genome.
Complete answer:
Difference between transformation and transduction are
Additional information:
There are a few similarities between transformation and transduction:
-Both are involved in horizontal gene transfer from one bacterium to another.
-A very amount of DNA is transferred by both mechanisms, and the entire chromosome content is not transferred.
-Both are important tools in molecular biology and genetic engineering.
Notes:
-Bacteriophages are viruses that have the capability to ingest inside bacteria and reproduce.
-Conjugation is an important method of DNA recombination, which works in association with transformation and transduction.
-Horizontal gene transfer is also known as lateral gene transfer is the mechanism of transfer of genetic material between non-related organisms.
Complete answer:
Difference between transformation and transduction are
Transformation | Transduction |
DNA fragments from a bacterium are passed and incorporated into the recipient’s chromosomes to cure the fault. | The transfer of DNA from the bacteria to the chromosome of the recipient requires a virus as an agent to carry the DNA fragment. |
It doesn’t require the presence of bacteriophage. | It requires the presence of bacteriophage. |
It doesn’t require any antibiotic resistance. | It requires antibiotic resistance. |
Plasmid transfer is possible. | Plasmid transfer is not possible. |
Naked DNA fragments move through the walls or membrane of the recipient. | It occurs through lytic or lysogenic cycles. |
It was discovered by Fred Griffith in 1928. | It was discovered by Norton Zinder and Joshua Lederberg in 1952. |
Transformation is susceptible to DNase. | Transduction is resistant to DNase. |
Additional information:
There are a few similarities between transformation and transduction:
-Both are involved in horizontal gene transfer from one bacterium to another.
-A very amount of DNA is transferred by both mechanisms, and the entire chromosome content is not transferred.
-Both are important tools in molecular biology and genetic engineering.
Notes:
-Bacteriophages are viruses that have the capability to ingest inside bacteria and reproduce.
-Conjugation is an important method of DNA recombination, which works in association with transformation and transduction.
-Horizontal gene transfer is also known as lateral gene transfer is the mechanism of transfer of genetic material between non-related organisms.
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