
Assertion (A): Virus-free plants can be produced from the virus-infected plant by means of meristem culture
Reason (R): Virus fails to grow during the growth of host tissue in the artificial medium
(a) A and R are true and R is the correct explanation of A
(b) A and R are true and R is not the correct explanation of A
(c) A is true, R is false
(d) A is false, R is true.
Answer
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Hint: In the event that the plant is tainted by the infection, the apical and axillary meristem will stay liberated from infection. Along these lines, one can eliminate the meristem and develop it in vitro to acquire infection-free plants.
Complete answer:
Infection doesn't develop during the development of host tissue in the counterfeit medium. This investigation was performed for the examination of meristem culture procedure with shoot tip culture strategy for acquiring infection-free plant, correlation of micropropagation accomplishment of two diverse supplement media, and assurance of the adequacy of constant PCR measure for the identification of infections.
Two distinctive garlic species (Allium sativum and Allium tuncelianum) and two diverse supplement media were utilized in this test. Results indicated that Medium 2 was more effective contrasted with Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone). In vitro plants obtained by means of meristem and shoot tip societies were tried for assurance of onion yellow bantam infection (OYDV) and leek yellow stripe infection (LYSV) through continuous PCR examination. In garlic plants spread by means of meristem culture, we were unable to recognize any infection.
So the correct answer is option C) A is true, R is false.
Note:
OYDV and LYSV infections were recognized in plants through shoot tip culture. OYDV infection was seen in measures of $80\%$ and $73\%$ of tried plants for A. tuncelianum and A. sativum, separately. LYSV infection was found in measure of $67\%$ of tried plants of A. tuncelianum and in measure of $87\%$ of tried plants of A. sativum in this investigation. This examination was directed at the Department of Horticulture, University of Çukurova Turkey, and Adana Veterinary Control Institute, Turkey between the years 2009 and 2010. Allium sativum (Kastamonu garlic clone) and Allium tuncelianum garlic species resolved to be contaminated with infections were utilized as plant material. A. sativum and A. tuncelianum garlic tests were given by Kastamonu-Taşköprü and Tunceli-Ovacık districts of Turkey, individually.
Complete answer:
Infection doesn't develop during the development of host tissue in the counterfeit medium. This investigation was performed for the examination of meristem culture procedure with shoot tip culture strategy for acquiring infection-free plant, correlation of micropropagation accomplishment of two diverse supplement media, and assurance of the adequacy of constant PCR measure for the identification of infections.
Two distinctive garlic species (Allium sativum and Allium tuncelianum) and two diverse supplement media were utilized in this test. Results indicated that Medium 2 was more effective contrasted with Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone). In vitro plants obtained by means of meristem and shoot tip societies were tried for assurance of onion yellow bantam infection (OYDV) and leek yellow stripe infection (LYSV) through continuous PCR examination. In garlic plants spread by means of meristem culture, we were unable to recognize any infection.
So the correct answer is option C) A is true, R is false.
Note:
OYDV and LYSV infections were recognized in plants through shoot tip culture. OYDV infection was seen in measures of $80\%$ and $73\%$ of tried plants for A. tuncelianum and A. sativum, separately. LYSV infection was found in measure of $67\%$ of tried plants of A. tuncelianum and in measure of $87\%$ of tried plants of A. sativum in this investigation. This examination was directed at the Department of Horticulture, University of Çukurova Turkey, and Adana Veterinary Control Institute, Turkey between the years 2009 and 2010. Allium sativum (Kastamonu garlic clone) and Allium tuncelianum garlic species resolved to be contaminated with infections were utilized as plant material. A. sativum and A. tuncelianum garlic tests were given by Kastamonu-Taşköprü and Tunceli-Ovacık districts of Turkey, individually.
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