Question

What are the materials used in observation of the nucleus in onion peel cells? And what colour of the nucellus will be observed under the microscope? And mention the shape of the nucleus in the plant cell?

Answer 1

Hint: Initially discovered by Hooke in 1665, the cell features a rich and interesting history that has ultimately given thanks to many of today's scientific advancements.

Complete answer:
An onion may be a multicellular (consisting of the many cells) plant organism. As altogether plant cells, the cell of an onion peel consists of a cell wall, cell membrane, cytoplasm, nucleus and an outsized vacuole.
Add a drop of methylthionine chloride over the slide. Wait for 2 minutes then remove the additional stain with blotter. Add a drop of glycerine on the slide. With the assistance of a needle, gently place the coverslip over the slide to avoid entry of air bubbles.
The cell wall encloses cytoplasm, cell organelles, and a nucleus. An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, light microscope, blotter, methylene blue (safranin) solution, glycerine, and water.
Precautions to be taken for temporary mount of onion peel are:
1. Use a brush to transfer onion peel to slip.
2. Avoid overstaining and understaining of the peel.
3. Avoid folding the peel while placing it on the slide.
4. Use a clean and dry glass slide and coverslip.
5. Avoid air bubbles while putting coverslip.

Note:
The cells of onion peel are rectangular in shape and that they have intercellular spaces between them. These cells have distinct cell walls.
The cells of onion peel have a linear or rectangular shape. Each cell features a prominent cell membrane, a nucleus and therefore the cytoplasm, encircling one or two large vacuoles.